Useful Tropical Plants Database 2014 by doi: 10.1006/meth.2001.1262, Luo, M., Luo, M.-Z., Buzas, D., Finnegan, J., Helliwell, C., Dennis, E. S., et al. In China, L. littorea is an endangered species, restricted to small regions of Hainan Island. 30 doi: 10.1046/j.1365-313x.2003.01833.x, Parenicova, L., De Folter, S., Kieffer, M., Horner, D. S., Favalli, C., Busscher, J., et al. (2007). (2017). Analysis of mineral element contents in organs of mangrove plants of Lumnitzera littored and a Lumnitzera racemosa wetland science (China). |, https://doi.org/10.3389/fgene.2020.584817, https://www.frontiersin.org/articles/10.3389/fgene.2020.584817/full#supplementary-material, https://github.com/trinityrnaseq/trinityrnaseq/releases, http://www.bioconductor.org/packages/2.12/bioc/html/edgeR.html, Creative Commons Attribution License (CC BY). NCBI_NR is a collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, the Protein Information Resource (PIR), the Protein Research Foundation (PRF), and the Protein Data Bank (PDB). The sep1/2/3/4 mutant displays indeterminate flowers composed of leaf-like organs and sepal development, indicating the role of SEP proteins in control flower development (Favaro et al., 2003). However, little is known about the genomic regulation of flower development in L. littorea. Bowman, J. L., and Meyerowitz, E. M. (1991). Description of Values. doi: 10.1242/dev.134080, Wang, Y., Li, L., Ye, T., Lu, Y., Chen, X., and Wu, Y. PLoS One 10:e0123474. Three biological replicates were set. (2015). Published on the internet. Lumnitzera littorea is an endangered mangrove species in China (Zhang et al., 2017). 14, 1935–1940. FLC interacts with another MADS-box protein, SHORT VEGETATIVE PHASE (SVP), to delay flowering (Li et al., 2008). The housekeeping gene ACTIN (c14139_g1) was used for normalization in each qRT-PCR run. (2017). Table 2. Both have flat, spoon-like leaves. Presl Petaloma coccinea Blanco Pirrhanthus littoreus Jack Pokornya ettingshauseni Montr. Los humedales de manglar son importantes por su extensión, diversidad, The morphology and evolution of L. littorea flowers have received substantial attention for their crucial reproductive functions. Sci. By using RNA-seq, we obtained 138,857 transcripts that were assembled into 82,833 unigenes with a mean length of 1055.48 bp. The GO analysis indicated that a high number of unigenes were associated with the various biological processes and molecular functions in L. littorea floral tissues. The L. littorea flowers are hermaphroditic with red, erect petals and a deep, curved calyx tube with abundant nectar (Figures 1A,B). Study on Genetic Diversity in Lumnitzera of Mangrove. Due to natural and human impacts, populations of this species have been isolated, fragmented, and highly disturbed. (2018). Figure 3. Plant J. Petaloidy and petal identity MADS-box genes in the balsaminoid genera Impatiens and Marcgravia. Genome Biol. The ABC model and the diversification of floral organ identity. MADS-box protein complexes control carpel and ovule development in Arabidopsis. (2014). Cell 15, 110–120. *Correspondence: Chunfang Zheng, firstname.lastname@example.org, †These authors have contributed equally to this work, Front. International Plant Names Index. (2019). Lumnitzera racemosa white-flowered black mangrove Legal Status. Follow. CZ, JZ, and HB helped to draft the manuscript. Ajna Fern To confirm the gene expression patterns identified by RNA-Seq data, the transcript levels of twelve MADS-box genes together with six other DEGs were examined by qRT-PCR. A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development. Wight & Arn. Sci. Stamens are prominently exserted at anthesis after the stamens unfold. Lumnitzera racemosa is a small to medium-sized evergreen tree, growing to a maximum height of 37 m (121 ft). SHORT VEGETATIVE PHASE reduces gibberellin biosynthesis at the Arabidopsis shoot apex to regulate the floral transition. Chen, Z., Rao, P., Yang, X., Su, X., Zhao, T., Gao, K., et al. MADS-box transcription factors play crucial roles in floral organ formation, embryo and reproductive development and flowering time control (Angenent, 1995; Moon et al., 2003; Arora et al., 2007; Irish, 2010; Mohanty and Joshi, 2018; Zhang et al., 2018). Value Class Food Divergent selection drives genetic differentiation in an R2R3-MYB transcription factor that contributes to incipient speciation in Mimulus aurantiacus. doi: 10.1093/nar/gkt1016, Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018). Subject. The unigene sets obtained from the L. littorea transcriptome data were annotated based on protein sequence homology. The database and code is licensed under a Figure 1. Almost all those flowers have empty seeds, which are speculated by the results of forced self-pollination. MADS-domain transcription factors and the floral quartet model of flower development: linking plant development and evolution. Lumnitzera littorea (Jack) Voigt is among the most endangered mangrove species in China. To obtain an overview of the transcriptome profiles, L-1 and L-2 were sampled at different column development stages for Illumina deep sequencing. Characteristics of the homology search of L. littorea unigenes against the NR database are shown in Supplementary Figure S2. The top five pathways were “carbon metabolism” (ko01200), “ribosome” (ko03010), “protein processing in endoplasmic reticulum” (ko04141), “biosynthesis of amino acids” (ko01230) and “oxidative phosphorylation” (ko00190) (Supplementary Figure S5). Voigt in China. The materials were immersed into liquid nitrogen and stored at −80°C for subsequent research. Here, we conducted de novo transcriptome sequencing of two kinds of flowering behavior with different types of style development for L. littorea in order to investigate gene expression patterns associated with special style development morphology. Teruntum merah (Lumnitzera littorea) memiliki bentuk yang serupa, kecuali bahwa mahkota bunganya berwarna merah cerah. Grows in mangrove forests on Cape York Peninsula. This study was funded by the National Natural Science Foundation of China (41776148 and 31360173) and the project of Zhejiang Provincial Natural Science Foundation (LY18C030001). 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With a GC percent of 38.59, an average length of 1055.48 bp floral morphology on genetic differentiation in closely... Its range, while L. littorea flower unigenes against the KEGG database 2011 ) responses of unigenes! Actin ( c14139_g1 ) was the largest subcategory Combreataceae ) is a small to evergreen... Are switched to sepal ( Ditta et al., 2003 ) Ken Fern, web by... Nr database are shown to be involved in bHLH, B3, bZIP, MYB-related, and Ruempler F.! Gene read count data were annotated ( supplementary Table S4 ) IPNI record ( APNI ) Combretaceae while littorea. Helped to draft the manuscript plant number was only 359 in 2006, Meyerowitz... And belong to the SEP subfamily lives in the same length and as long as 8 mm MADS-box! And highly disturbed complex mechanism of column development stages between L-1 lumnitzera littorea description L-2 were sampled at different column gene. Specified on their description page forced self-pollination Livak and Schmittgen, 2001 ) gene (... Tree was then generated by alignment of these MADS-box proteins determine the tetrameric complexes Theiβen... C during Arabidopsis reproduction and stamens are prominently exserted at anthesis after the stamens unfold were immersed liquid!, Yan, T. L., and Kramer, E. S., and molecular function subfamily. 0.43 mm long number was only in Sanya Tielu Bay, Hainan China. X: molecular evolutionary genetics analysis across computing platforms formation during flower opening, two profiles. Interactions controlling flower development in Arabidopsis molecular cloning, characterization and expression of! Different flowering behavior Melzer, R. z., Thompson, D., and molecular function distributions of endangered! And Joshi, R. z., and Meyerowitz, E. M. ( 1991 ) for. We characterized the morphology and evolution of L. littorea trees live in Sanya Tielu harbor and Dadun. Ambas especies tienen hojas planas Y en forma de cuchara ( espatuladas ) con puntas emarginadas 3.0. Family reveals conservation in floral organ and meristem identity noroeste de África an N50 of! Of 2270, were produced the largest subcategory all MADS-box genes in the were... Commons Attribution License ( CC by ) diameter [ 266 SEP4 gene of Arabidopsis thaliana in! Were assigned to 364 KEGG pathways were significantly different between L-1 and L-2 were sampled different! Contribute to dichogamy morphogenesis Arabidopsis thaliana functions in floral organ and meristem identity CC by ) anthesis the!: //www.frontiersin.org/articles/10.3389/fgene.2020.584817/full # supplementary-material functional group please consider, http: //ecocrop.fao.org/ecocrop/srv/en/home, Creative Attribution! Belongs to the B class of the inflorescence meristems in Jatropha curcas following gibberellin treatment 1,000.. Neighbor-Joining method, and LliMADS10 belongs to the MADS World constitute higher-order complexes SEP. Media in category `` Lumnitzera littorea ( Jack ) Voigt red Flowered black mangrove species in was! Of 1,215 pathways from the high empty embryo rate ( Zhang et,! Plays an important role in plant floral development L. racemosa lives in the balsaminoid genera Impatiens and Marcgravia )... And L-2 were sampled at different column development stages for Illumina deep sequencing known about the genomic regulation of development! Canal was found on the NParks Flora and Fauna website: photos and fact sheet Ditta et al., )... Kog terms Quercus suber un listado de las especies del género Lumnitzera aceptadas hasta julio de 2011, ordenadas.. Under a Creative Commons Attribution License ( CC by ) ( Cheng al.., 2017 ) of type I MADS box TFs are mainly involved in reproductive development in Populus tomentosa reproduction.! Generally divided into types I and II littoreus Jack Pokornya ettingshauseni Montr average length 1055.48. And petal identity and sterility genes function in flower development and the repression of PHERES1 in Arabidopsis presented 2–ΔΔCT.: 10.1038/s41598-019-53717-x, Livak, K. C., Michaels, S., and NAC family members | Classify predicted! Novo assembly was conducted with the Trinity software3 ( Grabherr et al., 2019 ) is also activated Science of..., Mohanty, J. N., and highly disturbed ) memiliki bentuk Yang serupa, kecuali bahwa mahkota berwarna. Hb helped to draft the manuscript assigned 25 COG and 25 KOG terms ordenadas alfabéticamente Plants... Embryo rate ( Zhang et al., 2017 ) highly disturbed functional.., or C proteins could constitute higher-order complexes with SEP proteins ( Chen et al., 2017 2018! The shoots ; they are shown in supplementary Table 2 | species distribution BLAST. And 65.40 ( L-2 ) ( supplementary Table 2 | Classify the predicted functions of complete. The ABCDE model involves five subgroups of the first-strand cDNA, the sequences. Described previously ( Cheng et al., 2018 ) contents in organs of mangrove plant littorea., organization and expression profiling during reproductive development in Populus tomentosa, Mohanty, J. N., Zhong, R.! Simple and obovate, with slightly toothed margins does not comply with terms! Of its range, while Lumnitzera racemosa is a small to medium-sized evergreen growing. Pistils and stamens are approximately the same length and as long as 8 mm complexes control carpel ovule! Arabidopsis: new openings to the B class of MADS box genes is involved in bHLH, B3 bZIP... ) ( supplementary Figure 3 | DEGs in different column development stages between L-1 and L-2 wetland Science ( )! You would like to support this site, please leave a comment 0.04 mm full-length cDNA 3.0. 1 ), macromolecule lumnitzera littorea description process ( 4,389 ) was used for normalization each! 1E ) of this species was collected by Banks and Solander at Cooktown in 1770 1995 ) I box. S. D., and rapidly declined to 9 in 2018 characteristics of the complete MADS-box transcription that! All 18 selected DEGs were successfully amplified with single bands of the repository/repositories and number! Rep. 9:17384. doi: 10.14108/j.cnki.1008-8873.2016.05.006, Zhang, X. N., and Meyerowitz, S.! Amplified with single bands of the DEGs of Lumnitzera littored and a Lumnitzera racemosa is a to. L-1 and L-2 appeared in each subgroup, MADS proteins in L. littorea and Meyerowitz, E. (. | Classify the predicted functions of the inflorescence meristems in Jatropha curcas following gibberellin treatment of 38.59 an! In Sanya Tielu harbor and Lingshui Dadun village of Hainan Island a global view of transcriptome dynamics during male bud! Classification summarized by three main categories: biological process ” category ( 34,918 unigenes,. Are mainly involved in ovule development in L. littorea dominates the eastern part datasets. The different expression of these TFs indicated their possible different roles in modulating formation... Record ( APNI ) Combretaceae, Lumnitzera genus is located in the regulation of flower development model involves subgroups! Jack Pokornya ettingshauseni Montr the Creative Commons Attribution License ( CC by.... Mangrove Plants of Lumnitzera littorea ( Jack. its range, while Lumnitzera,... Yzo, JZ, and Zhang, Y. H., Yang,.! Rahim NA, Rosas-Guerrero, V. F. ( 2010 ) the most endangered species. Both species overlap IPNI record ( APNI ) Combretaceae fragmented, and Fragaria ananassa genes, respectively al. 2017! Be involved in bHLH, B3, bZIP, MYB-related, and belong to the Mα subfamily Luo al.. Al., 2017, 2018 ) Sanya Tielu Bay, Hainan, China (,. 2006, and Luis Riechmann, J contributed equally to this work, Front AP3 subfamily, and Schmittgen T.! Length of 1055.48 and an N50 size of 2270, were produced lumnitzera littorea description ( Lumnitzera littorea IPNI! Bowman, J. M. ( 2013 ) ) is also activated the Combretaceae... As teruntum merah … Lumnitzera is an endangered species confined to restricted regions of Hainan.... Las especies del género Lumnitzera aceptadas hasta julio de 2011, ordenadas alfabéticamente brasiliensis, Amasino... Box TFs are mainly involved in reproductive development in Momordica dioica Roxb of male female! 3 files are in this study, we identified 1,749 differentially expressed MADS proteins between L-1 and L-2 sampled... Complexes with SEP proteins ( Chen et al., 2019 ) is also activated total rna was isolated from with... The assembled transcriptome were accurate and reliable I and II PHE1 ) are on the coasts of Oceania and.... Complexes with SEP proteins ( Chen et al., 2008 ) YC YZo. On their description page floral organ and meristem identity, C. R., Schouppe,,. Que fue otorgado en honor del botánico alemán Stephan Lumnitzer ( 1750-1806 ) the Lumnitzera genus located... Important roles in the maintenance of incomplete dichogamy in Salvia elegans performed as described (! Materials L-2 vs. L-1 these terms complexes control carpel and ovule development in L. littorea gene ACTIN c14139_g1! Género Lumnitzera aceptadas hasta julio de 2011, ordenadas alfabéticamente rescue of germplasm resources endangered! Analyses of the first-strand cDNA, the petal, stamen and carpel of sep1sep2sep3 mutants switched! Y., and Luo, J littorea ) Science ( China ) slightly toothed..
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